YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26787-1-AP

Synonyms

C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1

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Validation Data Gallery

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  WB analysis of HepG2 using 26787-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26787-1-AP (YTHDF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26787-1-AP

  IP result of anti-YTHDF1 (IP:26787-1-AP, 4ug; Detection:26787-1-AP 1:300) with HepG2 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Product Information

The immunogen of 26787-1-AP is YTHDF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDF1 fusion protein Ag25347
• Full Name: YTH domain family, member 1
• Calculated Molecular Weight: 559 aa, 61 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC050284
• Gene Symbol: YTHDF1
• Gene ID (NCBI): 54915
• RRID: AB_2880635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.

Publications

Species	Application	Title
human	WB,RIP,IHC	Cancer Res; RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis; Authors - Danyu Chen
mouse	WB,IHC	Cell Mol Neurobiol; Wnt3a/YTHDF1 Regulated Oxaliplatin-Induced Neuropathic Pain Via TNF-α/IL-18 Expression in the Spinal Cord; Authors - Xiaohui Bai
human	WB	Cancer Med; BIRC5 facilitates cisplatin-chemoresistance in a m6 A-dependent manner in ovarian cancer; Authors - Yadan Fan
human	WB	Cancer Cell Int; DUSP5 regulated by YTHDF1-mediated m6A modification promotes epithelial-mesenchymal transition and EGFR-TKI resistance via the TGF-β/Smad signaling pathway in lung adenocarcinoma; Authors - Weina Fan ; KD Validated