YOD1 Recombinant antibody

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  WB analysis using 84933-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84933-5-RR (YOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84933-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84933-5-RR against Human YOD1 were performed. The affinity constant is 1.07 nM.

YOD1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84933-5-RR

Clone No.242498G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84933-5-RR targets YOD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YOD1 fusion protein Ag21844
• Full Name: YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 348 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC137167
• Gene Symbol: YOD1
• Gene ID (NCBI): 55432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5VVQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YOD1, also known as YOD1 deubiquitinase, belongs to the ovarian tumor (OTU) domain-containing family of deubiquitinating enzymes (DUBs), which play a crucial role in regulating cellular processes by removing ubiquitin molecules from target proteins. YOD1 is particularly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it assists in the clearance of misfolded proteins from the endoplasmic reticulum (ER) by modulating the ubiquitination status of ERAD components. (PMID: 40132762,19818707)