YOD1 Polyclonal antibody

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  WB analysis using 25370-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25370-1-AP (YOD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25370-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25370-1-AP (YOD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 25370-1-AP

  IP result of anti-YOD1 (IP:25370-1-AP, 4ug; Detection:25370-1-AP 1:6000) with K-562 cells lysate 2400 ug.

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  IHC staining of human spleen using 25370-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25370-1-AP (YOD1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 25370-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25370-1-AP (YOD1 antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

YOD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 25370-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

DUBA 8, DUBA8, HIN 7, HIN7, HIV 1 induced protease 7, HsHIN7, OTUD2, PRO0907, Ubiquitin thioesterase OTU1, YOD1

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Product Information

25370-1-AP targets YOD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YOD1 fusion protein Ag21881
• Full Name: YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 348 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC137167
• Gene Symbol: YOD1
• Gene ID (NCBI): 55432
• RRID: AB_2880048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VVQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Lett; POH1 induces Smad3 deubiquitination and promotes lung cancer metastasis; Authors - Yang Yuan
mouse	WB	Comput Struct Biotechnol J; Identification of potential diagnostic and prognostic biomarkers for sepsis based on machine learning; Authors - Li Ke
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
human	WB	Cell Signal; Hypoxic tumor-derived exosomal miR-21 induces cancer-associated fibroblast activation to promote head and neck squamous cell carcinoma metastasis; Authors - Beibei Ye
human	WB,IHC	Cancer Cell Int; YOD1 serves as a potential prognostic biomarker for pancreatic cancer.; Authors - Zhishuo Zhang
human	WB	Biomed Res Int; Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway.; Authors - Xiao-Long Zhou ; KD Validated