YME1L1 Polyclonal antibody

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  WB analysis using 27856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27856-1-AP (YME1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 27856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27856-1-AP (YME1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using YME1L1 antibody (27856-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

YME1L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27856-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27856-1-AP targets YME1L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YME1L1 fusion protein Ag27280
• Full Name: YME1-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 716 aa, 80 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC023507
• Gene Symbol: YME1L1
• Gene ID (NCBI): 10730
• RRID: AB_3085999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96TA2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).