K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17150-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17150-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 17150-1-AP
IP result of anti-YARS2 (IP:17150-1-AP, 4ug; Detection:17150-1-AP 1:1000) with K-562 cells lysate 4400 ug.
Immunohistochemical analysis of paraffin-embedded human placenta using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 17150-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YARS2 antibody (17150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YARS2 antibody (17150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human testis tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
17150-1-AP targets YARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17150-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17150-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human testis using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 17150-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of K-562 using 17150-1-AP
IP result of anti-YARS2 (IP:17150-1-AP, 4ug; Detection:17150-1-AP 1:1000) with K-562 cells lysate 4400 ug.
IF/ICC Figures
IF Staining of HepG2 using 17150-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YARS2 antibody (17150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
YARS2 Polyclonal antibody
Catalog Number
17150-1-AP
Citations
-
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
