YARS Recombinant antibody

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  WB analysis using 84859-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84859-3-RR (YARS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84859-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84859-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 84859-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YARS antibody (84859-3-RR, Clone: 242400E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84859-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84859-3-RR against Human YARS were performed. The affinity constant is 0.414 nM.

YARS {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 84859-3-RR

Clone No.242400E9

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

YARS1, YTS, YRS, TYRRS, CMTDIC

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84859-3-RR targets YARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YARS fusion protein Ag35148
• Full Name: tyrosyl-tRNA synthetase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC016689
• Gene Symbol: YARS
• Gene ID (NCBI): 8565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54577
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)