YARS Polyclonal antibody

• 

  WB analysis using 31228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31228-1-AP (YARS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 31228-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 31228-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 31228-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 31228-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

YARS Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31228-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, ELISA

Synonyms

YARS1, YTS, YRS, TYRRS, CMTDIC

---

Tested Applications

• Positive WB detected in: A431 cells, U2OS cells, pig pancreas tissue, pig tonsil tissue
• Positive IHC detected in: human stomach cancer tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31228-1-AP targets YARS in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YARS fusion protein Ag35148
• Full Name: tyrosyl-tRNA synthetase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC016689
• Gene Symbol: YARS
• Gene ID (NCBI): 8565
• RRID: AB_3669909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P54577
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)