YAP1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

YAP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A7A9

Cat no : 66900-1-PBS

Synonyms

65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog

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Validation Data Gallery

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  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66900-1-Ig (same clone as 66900-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66900-1-Ig (same clone as 66900-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66900-1-Ig (same clone as 66900-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using YAP1 antibody (66900-1-Ig, Clone: 3A7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66900-1-PBS targets YAP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: YAP1 fusion protein Ag28194
• Full Name: Yes-associated protein 1, 65kDa
• Calculated Molecular Weight: 504 aa, 54 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC038235
• Gene Symbol: YAP1
• Gene ID (NCBI): 10413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). Phosphorylation of S381 primes YAP phosphorylation by CK1δ/ɛ, resulting in activation of a phosphodegron, thus generates a binding surface that interacts with a ubiquitin ligase, and leads to degradation by ubiquitination. (PMID: 20048001). The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65-70 kDa. (PMID: 26695440)