YAF2 Recombinant antibody

YAF2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240834G11

Cat no : 83798-6-RR

Synonyms

YY1-associated factor 2, YY1 associated factor 2, 240834G11

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Validation Data Gallery

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  WB analysis using 83798-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83798-6-RR (YAF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83798-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug YAF2 Recombinant antibody (83798-6-RR, Clone:240834G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: HEK-293T cells, Raji cells, HeLa cells, SK-N-SH cells, SH-SY5Y cells, human heart tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83798-6-RR is YAF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YAF2 fusion protein Ag5023
• Full Name: YY1 associated factor 2
• Calculated Molecular Weight: 204 aa, 23 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC037777
• Gene Symbol: YAF2
• Gene ID (NCBI): 10138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).