|Positive WB detected in||HEK-293T cells, COLO 320 cells, HEK-293 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
11267-1-AP targets XRN2 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||XRN2 fusion protein Ag1788|
|Full Name||5'-3' exoribonuclease 2|
|Calculated molecular weight||104 kDa|
|Observed molecular weight||104 kDa|
|GenBank accession number||BC006417|
|Gene ID (NCBI)||22803|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII.
Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids.
J Cell Biol
Intronless mRNAs transit through nuclear speckles to gain export competence.
Characterization of miR-122-independent propagation of HCV.
Dissecting the roles of the 5' exoribonucleases xrn1 and xrn2 in restricting hepatitis C virus replication.