XRN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

XRN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23108-1-AP

Synonyms

5 3 exoribonuclease 1, 5' 3' exoribonuclease 1, SEP1, XRN1

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Validation Data Gallery

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  WB analysis of HeLa using 23108-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23108-1-AP (XRN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 23108-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23108-1-AP (XRN1 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 23108-1-AP

  Immunofluorescent analysis of HeLa cells using 23108-1-AP (XRN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human heart tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

23108-1-AP targets XRN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XRN1 fusion protein Ag19398
• Full Name: 5'-3' exoribonuclease 1
• Calculated Molecular Weight: 1706 aa, 194 kDa
• Observed Molecular Weight: 175 kDa
• GenBank Accession Number: BC078146
• Gene Symbol: XRN1
• Gene ID (NCBI): 54464
• RRID: AB_2879211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Exoribonuclease I (XRN1), also known as Sep1 or Rar5, is a 1,694-amino acid protein that functions as the major cytoplasmic 5 prime to 3 prime exoribonuclease and plays an important role in mRNA turnover. XRN1 may also function in the microtubular cytoskeleton as well as in DNA recombination and replication. XRN1 induces the expression of stress granules (SGs), cytoplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress, and GW bodies/processing bodies (PBs), distinct cytoplasmic sites of mRNA degradation. There are several isoforms of XRN1 that are produced as a result of alternative splicing events.Loss of XRN1 markedly affects cell growth rates.This antibody is specific to the XRN1 protein.

Publications

Species	Application	Title
human	WB	Cancer Res; NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer; Authors - Ruihui Xie
human	WB	Nucleic Acids Res; LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential; Authors - Damian M Janecki ; KD Validated