Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, COLO 320 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, rat testis tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15817-1-AP targets XRCC4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8606 Product name: Recombinant human XRCC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-310 aa of BC005259 Sequence: SRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKEKGRKKETSEKEAV Predict reactive species |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 4 |
| Calculated Molecular Weight | 310 aa, 35 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC005259 |
| Gene Symbol | XRCC4 |
| Gene ID (NCBI) | 7518 |
| RRID | AB_2257478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13426 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for XRCC4 antibody 15817-1-AP | Download protocol |
| IP protocol for XRCC4 antibody 15817-1-AP | Download protocol |
| WB protocol for XRCC4 antibody 15817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-XRCC4 antibody (Cat# 15817-1-AP) has 17 citations published in journals including Nature Communications, Cancer Research, Cell Death & Differentiation, Cell Death & Disease, PLoS Biology, Journal of Translational Medicine, Phytomedicine, Cell Signaling, FEBS Journal, FEBS Letters, Bioscience Reports, Toxicology & Applied Pharmacology, Journal of Cancer, PLoS ONE, Nutrition & Cancer, and Oncology Letters. Research fields span DNA repair (NHEJ), cancer biology, radiosensitivity, and immune signaling.
| Species | Application | Title |
|---|---|---|
Cancer Res Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining. | ||
Nat Commun Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.
| ||
Nat Commun Elevated nonhomologous end-joining by AATF enables efficient DNA damage repair and therapeutic resistance in glioblastoma | ||
Cell Death Differ LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair. | ||
Cell Death Dis Intermediate filament IFFO1 negatively regulates the migration of lung cancer cells by inhibiting the IQGAP3-Cdc42 interaction | ||
Phytomedicine Guiqi Baizhu decoction enhances radiosensitivity in non-small cell lung cancer by inhibiting the HIF-1α/DNA-PKcs axis-mediated DNA repair |
Reviews
What customers say
Users report consistently positive experiences with this antibody. Two reviewers highlighted excellent performance in ChIP-seq on U2OS cells, praising reproducible results at a 1:250 dilution. Another reviewer tested it across Western Blot, Immunofluorescence, and Immunoprecipitation, finding it effective for plasmid-expressed targets at 1000x dilution while recommending 500x for detecting endogenous XRCC4. Overall, the antibody is well-regarded for its reliability and reproducibility across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mohammed (Verified Customer) (05-20-2026) | The product is good and gives reproducible ChIP-Seq results.
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Mohammed (Verified Customer) (04-22-2026) | The antibody works perfect for ChIP-seq and it gives reporducible results
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Alexandra (Verified Customer) (07-02-2024) | For plasmid expression it works fine with 1000x dilution, but for the detection of endogenous XRCC4 I recommend the use of 500x dilution.
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