XRCC3 Monoclonal antibody, PBS Only

XRCC3 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12F9

Cat no : 67222-1-PBS

Synonyms

DNA repair protein XRCC3, XRCC3

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Validation Data Gallery

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  WB analysis using 67222-1-Ig (same clone as 67222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 67222-1-Ig (same clone as 67222-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 67222-1-Ig (same clone as 67222-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 67222-1-Ig (same clone as 67222-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 67222-1-Ig (same clone as 67222-1-PBS)

  IP result of anti-XRCC3 (IP:67222-1-Ig, 5ug; Detection:67222-1-Ig 1:500) with MCF-7 cells lysate 640 ug. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67222-1-Ig (same clone as 67222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67222-1-Ig (same clone as 67222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67222-1-PBS targets XRCC3 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: XRCC3 fusion protein Ag13317
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 3
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC011725
• Gene Symbol: XRCC3
• Gene ID (NCBI): 7517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.