XPR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

XPR1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14174-1-AP

Synonyms

X receptor, SYG1, Solute carrier family 53 member 1, SLC53A1, Protein SYG1 homolog

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Validation Data Gallery

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  WB analysis of Jurkat using 14174-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14174-1-AP (XPR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14174-1-AP (XPR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14174-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14174-1-AP (XPR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14174-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14174-1-AP (XPR1 antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of Jurkat using 14174-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug XPR1 Polyclonal antibody (14174-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14174-1-AP targets XPR1 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XPR1 fusion protein Ag5373
• Full Name: xenotropic and polytropic retrovirus receptor
• Calculated Molecular Weight: 696 aa, 82 kDa
• Observed Molecular Weight: 82-84 kDa
• GenBank Accession Number: BC041142
• Gene Symbol: XPR1
• Gene ID (NCBI): 9213
• RRID: AB_10642569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Control of XPR1-dependent cellular phosphate efflux by InsP8 is an exemplar for functionally-exclusive inositol pyrophosphate signaling.; Authors - Xingyao Li ; KO Validated
human	WB	Cancer Sci; PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer; Authors - Yoko Akasu-Nagayoshi ; KD Validated
human	WB	Biochem Biophys Res Commun; Golgi damage caused by dysfunction of PiT-2 in primary familial brain calcification; Authors - Huifang Sun
rat	WB	J Med Invest; Effects of EOS789, a novel pan-phosphate transporter inhibitor, on phosphate metabolism : Comparison with a conventional phosphate binder; Authors - Kazuya Tanifuji
human,mouse	WB,IHC	Cancer Cell Int; CircGNB1 facilitates the malignant phenotype of GSCs by regulating miR-515-5p/miR-582-3p-XPR1 axis; Authors - Jinpeng Hu
human	WB	Cell Rep; Homeostatic coordination of cellular phosphate uptake and efflux requires an organelle-based receptor for the inositol pyrophosphate IP8; Authors - Xingyao Li