XK Polyclonal antibody

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  WB analysis of mouse pancreas using 12830-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12830-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 40x lens).

XK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12830-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Kell complex 37 kDa component, KX, Kx antigen, Membrane transport protein XK, X1k, XK, XK related protein 1, XKR1, XRG1

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12830-1-AP targets XK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XK fusion protein Ag3550
• Full Name: X-linked Kx blood group (McLeod syndrome)
• Calculated Molecular Weight: 444 aa, 51 kDa
• Observed Molecular Weight: 45-51 kDa
• GenBank Accession Number: BC036019
• Gene Symbol: XK
• Gene ID (NCBI): 7504
• RRID: AB_2877887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51811
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XK, also named as XKR1 and XRG1, is responsible for the Kx blood group system. XK forms a functional complex with the Kell glycoprotein. It represents a crucial factor for the maintenance of normal muscle structure and function.