• Featured Product
  • KD/KO Validated

XIAP Monoclonal antibody

XIAP Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A1A12

Cat no : 66800-1-Ig

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2



Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells
Positive IHC detected inhuman colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue, human colon tissue
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66800-1-Ig targets XIAP in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen XIAP fusion protein Ag18088
Full Name X-linked inhibitor of apoptosis
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC032729
Gene Symbol XIAP
Gene ID (NCBI) 331
RRIDAB_2882143
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.

Protocols

Product Specific Protocols
WB protocol for XIAP antibody 66800-1-IgDownload protocol
IHC protocol for XIAP antibody 66800-1-IgDownload protocol
IF protocol for XIAP antibody 66800-1-IgDownload protocol
FC protocol for XIAP antibody 66800-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Inhibition of PI3K/AKT signaling via ROS regulation is involved in Rhein-induced apoptosis and enhancement of oxaliplatin sensitivity in pancreatic cancer cells.

Authors - Yuhui Liu
humanWB

Oncol Lett

PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p-eIF4B/XIAP signaling pathway in prostate cancer.

Authors - Siwen Yin
humanWB

bioRxiv

Recruitment of FBXO22 for Targeted Degradation of NSD2

Authors - David Y Nie
humanWB

Gene

Study on the mechanism of S100A4-mediated cancer oncogenesis in uveal melanoma cells through the integration of bioinformatics and in vitro experiments

Authors - Huimei Chen
humanWB

Nat Chem Biol

Recruitment of FBXO22 for targeted degradation of NSD2

Authors - David Y Nie
humanWB

Chem Biol Interact

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65

Authors - Jingwen Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Praveen (Verified Customer) (05-17-2019)

Very Good Antibody

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: AC16
XIAP Antibody Immunoprecipitation, validation ( dilution) in AC16 (Cat no:66800-1-Ig)
FH

Praveen (Verified Customer) (05-17-2019)

Very good Antibody

  • Applications: Immunoprecipitation,
XIAP Antibody Immunoprecipitation, validation ( dilution) in (Cat no:66800-1-Ig)