XIAP Monoclonal antibody

• Featured Product
• KD/KO Validated

XIAP Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

2A1A12

Reviews (2)

Cat no : 66800-1-Ig

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2

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Validation Data Gallery

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  WB analysis using 66800-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HEK-293 using 66800-1-Ig

  WB result of XIAP antibody (66800-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XIAP transfected HEK-293 cells.

• 

  WB analysis using 66800-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 66800-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66800-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66800-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66800-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66800-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66800-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66800-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon using 66800-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon using 66800-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66800-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human XIAP (66800-1-Ig, Clone:2A1A12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells
• Positive IHC detected in: human colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon cancer tissue, human colon tissue
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66800-1-Ig targets XIAP in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: XIAP fusion protein Ag18088
• Full Name: X-linked inhibitor of apoptosis
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC032729
• Gene Symbol: XIAP
• Gene ID (NCBI): 331
• RRID: AB_2882143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Inhibition of PI3K/AKT signaling via ROS regulation is involved in Rhein-induced apoptosis and enhancement of oxaliplatin sensitivity in pancreatic cancer cells.; Authors - Yuhui Liu
human	WB	Oncol Lett; PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p-eIF4B/XIAP signaling pathway in prostate cancer.; Authors - Siwen Yin
human	WB	bioRxiv; Recruitment of FBXO22 for Targeted Degradation of NSD2; Authors - David Y Nie
human	WB	Gene; Study on the mechanism of S100A4-mediated cancer oncogenesis in uveal melanoma cells through the integration of bioinformatics and in vitro experiments; Authors - Huimei Chen
human	WB	Nat Chem Biol; Recruitment of FBXO22 for targeted degradation of NSD2; Authors - David Y Nie
human	WB	Chem Biol Interact; Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65; Authors - Jingwen Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Praveen (Verified Customer) (05-17-2019)

Very Good Antibody

• Applications: Immunoprecipitation,
• Cell Tissue Type: AC16

XIAP Antibody Immunoprecipitation, validation ( dilution) in AC16 (Cat no:66800-1-Ig)

FH

Praveen (Verified Customer) (05-17-2019)

Very good Antibody

• Applications: Immunoprecipitation,

XIAP Antibody Immunoprecipitation, validation ( dilution) in (Cat no:66800-1-Ig)