XIAP Polyclonal antibody

XIAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 23453-1-AP

Synonyms

IAP-3, hIAP-3, EC:2.3.2.27, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4

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Validation Data Gallery

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  WB analysis using 23453-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23453-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 23453-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23453-1-AP (XIAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23453-1-AP (XIAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 23453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 23453-1-AP (XIAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells, Tunicamycin treated HepG2 cells, HEK-293 cells, NIH/3T3 cells
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

23453-1-AP targets XIAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XIAP fusion protein Ag0725
• Full Name: X-linked inhibitor of apoptosis
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC032729
• Gene Symbol: XIAP
• Gene ID (NCBI): 331
• RRID: AB_2879283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56kd.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.; Authors - Ruqi Jiang
human	WB,IF	Biochim Biophys Acta Mol Cell Res; HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway; Authors - Ziyuan Tong
human	WB	Front Immunol; Metabolic Reprogramming Mediates Delayed Apoptosis of Human Neutrophils Infected With Francisella tularensis.; Authors - Samantha J Krysa
human	WB	Environ Pollut; DBDPE upregulates NOD-like receptor signaling to induce NLRP3 inflammasome-mediated HAECs pyroptosis; Authors - Li Jing