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  • KD/KO Validated

WWP2 Polyclonal antibody

WWP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12197-1-AP

Synonyms

AIP2, WW domain containing protein 2, WWP2, WWp2 like



Tested Applications

Positive WB detected inHepG2 cells, A549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12197-1-AP targets WWP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WWP2 fusion protein Ag2835
Full Name WW domain containing E3 ubiquitin protein ligase 2
Calculated Molecular Weight 870 aa, 99 kDa
Observed Molecular Weight 110-115 kDa
GenBank Accession NumberBC013645
Gene Symbol WWP2
Gene ID (NCBI) 11060
RRIDAB_10597536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WWP2(WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes(PMID:21532586).WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells(PMID:15047715).

Protocols

Product Specific Protocols
WB protocol for WWP2 antibody 12197-1-APDownload protocol
IHC protocol for WWP2 antibody 12197-1-APDownload protocol
IP protocol for WWP2 antibody 12197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

Cardiovasc Diabetol

Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X

Authors - Shilong You
  • KD Validated
humanWB

Cell Death Differ

Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.

Authors - Naijin Zhang
  • KD Validated
mouseWB

Redox Biol

Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury.

Authors - Naijin Zhang
humanWB

Br J Pharmacol

Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.

Authors - Mao-Xu Ge
mouseWB,IHC,IF

J Dent Res

WWP2 Promotes Odontoblastic Differentiation by Monoubiquitinating KLF5.

Authors - J Fu
mouseWB

J Cell Mol Med

WWP2 regulates SIRT1-STAT3 acetylation and phosphorylation involved in hypertensive angiopathy.

Authors - Ying Zhang