WWP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

WWP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 12197-1-AP

Synonyms

AIP2, WW domain containing protein 2, WWP2, WWp2 like

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Validation Data Gallery

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  WB analysis of HepG2 using 12197-1-AP

  WB result of WWP2 antibody (12197-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWP2 transfected HepG2 cells.

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  WB analysis of A549 using 12197-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12197-1-AP (WWP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12197-1-AP

  IP result of anti-WWP2 (IP:12197-1-AP, 4ug; Detection:12197-1-AP 1:1000) with A549 cells lysate 800 ug.

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  IHC staining of human pancreas cancer using 12197-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12197-1-AP (WWP2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12197-1-AP targets WWP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WWP2 fusion protein Ag2835
• Full Name: WW domain containing E3 ubiquitin protein ligase 2
• Calculated Molecular Weight: 870 aa, 99 kDa
• Observed Molecular Weight: 110-115 kDa
• GenBank Accession Number: BC013645
• Gene Symbol: WWP2
• Gene ID (NCBI): 11060
• RRID: AB_10597536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WWP2(WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes(PMID:21532586).WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells(PMID:15047715).

Publications

Species	Application	Title
	WB,IF	Cardiovasc Diabetol; Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X; Authors - Shilong You ; KD Validated
human	WB	Cell Death Differ; Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.; Authors - Naijin Zhang ; KD Validated
mouse	WB	Redox Biol; Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury.; Authors - Naijin Zhang
human	WB	Br J Pharmacol; Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.; Authors - Mao-Xu Ge
mouse	WB,IHC,IF	J Dent Res; WWP2 Promotes Odontoblastic Differentiation by Monoubiquitinating KLF5.; Authors - J Fu
mouse	WB	J Cell Mol Med; WWP2 regulates SIRT1-STAT3 acetylation and phosphorylation involved in hypertensive angiopathy.; Authors - Ying Zhang