WWP1 Monoclonal antibody, PBS Only

WWP1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G8G11

Cat no : 67804-1-PBS

Synonyms

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

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Validation Data Gallery

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  WB analysis using 67804-1-Ig (same clone as 67804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67804-1-PBS in a different storage buffer formulation.

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  WB analysis of HaCaT using 67804-1-Ig (same clone as 67804-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67804-1-PBS in a different storage buffer formulation.

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Product Information

67804-1-PBS targets WWP1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: WWP1 fusion protein Ag30171
• Full Name: WW domain containing E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 922 aa, 105 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC036065
• Gene Symbol: WWP1
• Gene ID (NCBI): 11059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.