WWP1 Polyclonal antibody

WWP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28689-1-AP

Synonyms

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

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Validation Data Gallery

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  WB analysis of HepG2 using 28689-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28689-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 28689-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 28689-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, MCF-7 cells, A431 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

28689-1-AP targets WWP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WWP1 fusion protein Ag30161
• Full Name: WW domain containing E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 922 aa, 105 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC036065
• Gene Symbol: WWP1
• Gene ID (NCBI): 11059
• RRID: AB_2881195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cancer Med; Quercetin-induced degradation of RhoC suppresses hepatocellular carcinoma invasion and metastasis; Authors - Chunlong Huang
human	WB,IHC	Cell Mol Biol Lett; Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis; Authors - Wei Jin