WWP1 Polyclonal antibody

• 

  WB analysis of MCF-7 using 13587-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13587-1-AP (WWP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13587-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13587-1-AP (WWP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

WWP1 Polyclonal Antibody for ELISA

Cat No. 13587-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AIP5, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

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Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Product Information

13587-1-AP targets WWP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WWP1 fusion protein Ag3582
• Full Name: WW domain containing E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 922 aa, 105 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC036065
• Gene Symbol: WWP1
• Gene ID (NCBI): 11059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0M0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Elife; Sushi domain-containing protein 4 controls synaptic plasticity and motor learning.; Authors - Inés González-Calvo
human	WB,IHC	Oncotarget; Overexpression of WWP1 promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma.; Authors - Xiao-Fei Zhang ; KD Validated
human	WB	Mol Oncol; Protein kinase CK2 activation is required for transforming growth factor β-induced epithelial-mesenchymal transition.; Authors - Seongrak Kim
human	WB	Oncotarget; The eIF2α serine 51 phosphorylation-ATF4 arm promotes HIPPO signaling and cell death under oxidative stress.; Authors - Kamindla Rajesh
human, mouse	WB	J Biol Chem; E3 ubiquitin ligase, WWP1, interacts with AMPKα2 and down-regulates its expression in skeletal muscle C2C12 cells.; Authors - Lee Jung Ok JO
human	WB	Exp Cell Res; NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.; Authors - Jianning Xu