WWOX Monoclonal antibody, PBS Only

WWOX Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8F4

Cat no : 67725-1-PBS

Synonyms

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX

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Validation Data Gallery

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  WB analysis using 67725-1-Ig (same clone as 67725-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67725-1-Ig (WWOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67725-1-Ig (same clone as 67725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67725-1-Ig (WWOX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

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Product Information

67725-1-PBS targets WWOX in WB, IHC, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

• Tested Reactivity: Human, rat, pig, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: WWOX fusion protein Ag7969
• Full Name: WW domain containing oxidoreductase
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC003184
• Gene Symbol: WWOX
• Gene ID (NCBI): 51741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.