WWOX Monoclonal antibody, PBS Only (Detector)

• 

  WB analysis using 67725-1-Ig (same clone as 67725-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67725-1-Ig (WWOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67725-1-Ig (same clone as 67725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67725-1-Ig (WWOX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51258-1

  Cytometric bead array standard curve of MP51258-1, WWOX Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67725-2-PBS. Detection antibody: 67725-1-PBS. Standard:Ag7969. Range: 0.195-100 ng/mL.

WWOX Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 67725-1-PBS

Clone No.1B8F4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

FRA16D, FOR1, FOR, EC:1.1.1.-, D16S432E

---

Product Information

67725-1-PBS targets WWOX as part of a matched antibody pair:

MP51258-1: 67725-2-PBS capture and 67725-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: WWOX fusion protein Ag7969
• Full Name: WW domain containing oxidoreductase
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC003184
• Gene Symbol: WWOX
• Gene ID (NCBI): 51741
• RRID: AB_2882911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZC7
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.