WTAP Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

WTAP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10200-1-PBS

Synonyms

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, Mum2, KIAA0105, FL2D

---

Validation Data Gallery

• 

  WB analysis of HeLa using 10200-1-AP (same clone as 10200-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10200-1-AP (same clone as 10200-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat cells using 10200-1-AP (same clone as 10200-1-PBS)

  WB result of WTAP specific antibody (10200-1-AP, 1:6000) with si-control and si-WTAP transfected Jurkat cells. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10200-1-AP (same clone as 10200-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10200-1-AP (same clone as 10200-1-PBS)

  IP result of anti-WTAP (IP:10200-1-AP, 3ug; Detection:10200-1-AP 1:500) with HEK-293 cells lysate 2500ug. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 10200-1-AP (same clone as 10200-1-PBS)

  IP result of anti-WTAP (IP:10200-1-AP, 4ug; Detection:10200-1-AP 1:8000) with Jurkat cells lysate 2280 ug. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10200-1-AP (same clone as 10200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 10200-1-AP (same clone as 10200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

---

Product Information

10200-1-PBS targets WTAP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WTAP fusion protein Ag0259
• Full Name: Wilms tumor 1 associated protein
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000383
• Gene Symbol: WTAP
• Gene ID (NCBI): 9589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.