WT1 Recombinant antibody

WT1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2J13

Cat no : 82525-1-RR

Synonyms

Wilms tumor protein, Wilms tumor 1, 2J13

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Validation Data Gallery

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  WB analysis using 82525-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82525-1-RR (WT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 82525-1-RR

  IP result of anti-WT1 (IP:82525-1-RR, 4ug; Detection:82525-1-RR 1:1000) with K-562 cells lysate 1600 ug.

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  IHC staining of rat testis using 82525-1-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82525-1-RR (WT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 82525-1-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82525-1-RR (WT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82525-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82525-1-RR against Human WT1 were performed. The affinity constant is 7.19 nM.

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82525-1-RR targets WT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WT1 fusion protein Ag19541
• Full Name: Wilms tumor 1
• Calculated Molecular Weight: 449 aa, 49 kDa, 57 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC032861
• Gene Symbol: WT1
• Gene ID (NCBI): 7490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.