WSTF Monoclonal antibody

WSTF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F9F12

Cat no : 68361-1-Ig

Synonyms

BAZ1B, hWALp2, Tyrosine protein kinase BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF

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Validation Data Gallery

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  WB analysis using 68361-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68361-1-Ig (BAZ1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U2OS cells, T-47D cells, MDA-MB-231 cells, SK-BR-3 cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68361-1-Ig targets WSTF in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WSTF fusion protein Ag24403
• Full Name: bromodomain adjacent to zinc finger domain, 1B
• Calculated Molecular Weight: 1483 aa, 171 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC136520
• Gene Symbol: WSTF/BAZ1B
• Gene ID (NCBI): 9031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tyrosine-protein kinase BAZ1B, also named WSTF, plays a central role in chromatin remodeling and acts as a transcription regulator (PMID:19092802). It involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph) (PMID:19234442).