WRAP53 Polyclonal antibody

• Featured Product
• KD/KO Validated

WRAP53 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 14761-1-AP

Synonyms

TCAB1, WDR79, WRAP53

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Validation Data Gallery

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  WB analysis using 14761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14761-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14761-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14761-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14761-1-AP

  IP result of anti-WRAP53 (IP:14761-1-AP, 4ug; Detection:14761-1-AP 1:300) with HeLa cells lysate 2480ug.

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Raji cells, human kidney tissue, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

14761-1-AP targets WRAP53 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WRAP53 fusion protein Ag6522
• Full Name: WD repeat containing, antisense to TP53
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 72-75 kDa
• GenBank Accession Number: BC002336
• Gene Symbol: WRAP53
• Gene ID (NCBI): 55135
• RRID: AB_1939806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Genes Dev; The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.; Authors - Sofia Henriksson
human	WB	Nat Chem Biol; Observation of processive telomerase catalysis using high-resolution optical tweezers.; Authors - Eric M Patrick
human	WB	Oncogenesis; RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.; Authors - Qunsong Tan ; KD Validated
	WB	Aging (Albany NY); Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.; Authors - Xing-Hua Pan
human	WB,IF	RNA Biol; Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.; Authors - Christos Coucoravas ; KD Validated
human	WB	Cancer Cell Int; Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.; Authors - Jing Niu