WNT8A Polyclonal antibody, PBS Only

WNT8A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30518-1-PBS

Synonyms

WNT8D, Protein Wnt-8d, Protein Wnt-8a, Protein Wnt 8d, Protein Wnt 8a

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Validation Data Gallery

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  WB analysis using 30518-1-AP (same clone as 30518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30518-1-AP (WNT8A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30518-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 30518-1-AP (same clone as 30518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30518-1-AP (WNT8A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 30518-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 30518-1-AP (same clone as 30518-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using WNT8A antibody (30518-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30518-1-PBS in a different storage buffer formulation.

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Product Information

30518-1-PBS targets WNT8A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT8A fusion protein Ag29510
• Full Name: wingless-type MMTV integration site family, member 8A
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: NM_001300938
• Gene Symbol: WNT8A
• Gene ID (NCBI): 7478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.