WNT4 Polyclonal antibody

WNT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14371-1-AP

Synonyms

WNT 4, UNQ426/PRO864, SERKAL, Protein Wnt-4, Protein Wnt 4

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Validation Data Gallery

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  WB analysis using 14371-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14371-1-AP (WNT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 14371-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human kidney tissue, human hepatocirrhosis tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

14371-1-AP targets WNT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT4 fusion protein Ag5741
• Full Name: wingless-type MMTV integration site family, member 4
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC057781
• Gene Symbol: WNT4
• Gene ID (NCBI): 54361
• RRID: AB_2215428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNT4 belongs to the Wnt family. WNT4 is critical for embryonic organogenesis, but later plays a unique role among Wnt proteins in the development and maintenance of müllerian and reproductive tissues (PMID: 33963381). The mesenchymal stem cells can improve cardiac function after cardiac injury by regulating endothelial cells via the Wnt4/β-catenin signaling pathway (PMID: 20551912). WNT4 may be modified by glycosylation, leading to an increase in molecular weight.

Publications

Species	Application	Title
human	WB	Endocrinology; PMID: 31748790; Authors - Alison M Neff
rat		Int J Clin Exp Pathol; Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1.; Authors - Junyi Hua
mouse	IHC	Neurochem Res; Wnt Signaling is Altered by Spinal Cord Neuronal Dysfunction in Amyotrophic Lateral Sclerosis Transgenic Mice.; Authors - Yu Li L
human	IF	Cell Death Dis; The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.; Authors - Nanchao Hong
mouse	IHC	BMC Complement Med Ther; Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.; Authors - Bing Cao
human	WB,IHC	Eur J Pharmacol; HucMSC exosomes attenuate partial bladder outlet obstruction-induced renal injury and cell proliferation via the Wnt/β-catenin pathway; Authors - Zhaoying Wang