WNT3A Recombinant antibody, PBS Only (Capture)

WNT3A Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241993D6

Cat no : 84577-5-PBS

Synonyms

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP01423-3

  Sandwich ELISA standard curve of MP01423-3, Human WNT3A Recombinant Matched Antibody Pair - PBS only. 84577-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25051. 84577-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01423-3

  The mean WNT3A concentration was determined to be 999.1 pg/mL in MDA-MB-231 cell extract based on a 1.2 mg/mL extract load and 2,063.4 pg/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

• 

  Sample test of MP01423-3

  Serum of six individual healthy human donors was measured. The WNT3A concentration of detected samples was determined to be 2,012.8 pg/mL with a range of 101.5-6,680.8 pg/mL.

---

Product Information

84577-5-PBS targets WNT3A as part of a matched antibody pair:

MP01423-3: 84577-5-PBS capture and 84577-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WNT3A fusion protein Ag25051
• Full Name: wingless-type MMTV integration site family, member 3A
• Calculated Molecular Weight: 352 aa, 39 kDa
• GenBank Accession Number: BC103921
• Gene Symbol: WNT3A
• Gene ID (NCBI): 89780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.