WNT2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

WNT2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H7B10

Cat no : 66656-1-PBS

Synonyms

Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

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Validation Data Gallery

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  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66656-1-Ig (same clone as 66656-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66656-1-Ig (same clone as 66656-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66656-1-Ig (same clone as 66656-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66656-1-Ig (WNT2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66656-1-PBS targets WNT2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WNT2 fusion protein Ag25185
• Full Name: wingless-type MMTV integration site family member 2
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC029854
• Gene Symbol: WNT2
• Gene ID (NCBI): 7472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.