WNT2 Monoclonal antibody

• Featured Product
• KD/KO Validated

WNT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

3H7B10

Cat no : 66656-1-Ig

Synonyms

Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

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Validation Data Gallery

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  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of RAW 264.7 using 66656-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66656-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 66656-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66656-1-Ig (WNT2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, Jurkat cells, RAW 264.7 cells, rat brain tissue, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

66656-1-Ig targets WNT2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WNT2 fusion protein Ag25185
• Full Name: wingless-type MMTV integration site family member 2
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC029854
• Gene Symbol: WNT2
• Gene ID (NCBI): 7472
• RRID: AB_2882013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

Publications

Species	Application	Title
human	WB,IF	Sci Adv; Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer; Authors - Atish Mohanty ; KD Validated
human	IF	Cell Mol Life Sci; α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway.; Authors - Zewu Zhu
human	WB	J Surg Res; Expression Analysis of ACSL5 and Wnt2B in Human Congenital Pulmonary Airway Malformations.; Authors - Yuan Qu
rat	WB	Int J Endocrinol; Fufang Xueshuantong Improves Diabetic Cardiomyopathy by Regulating the Wnt/β-Catenin Pathway; Authors - Meizhong Peng
human	WB	Int J Oncol; FUT2 promotes the tumorigenicity and metastasis of colorectal cancer cells via the Wnt/β‑catenin pathway; Authors - Peng Liu
rat	WB	Biochim Biophys Acta Mol Cell Res; Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB; Authors - Weilin Fang