WIPF2 Polyclonal antibody

WIPF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20512-1-AP

Synonyms

WICH, WIP related protein, WIPF2, WIRE

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 20512-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20512-1-AP (WIPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20512-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20512-1-AP (WIPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 20512-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20512-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, mouse colon tissue
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20512-1-AP is WIPF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WIPF2 fusion protein Ag14330
• Full Name: WAS/WASL interacting protein family, member 2
• Calculated Molecular Weight: 440 aa, 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC065551
• Gene Symbol: WIPF2
• Gene ID (NCBI): 147179
• RRID: AB_10695185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.