WIF1 Monoclonal antibody

WIF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2G6A3

Cat no : 67904-1-Ig

Synonyms

WIF 1, WIF1, WNT inhibitory factor 1

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Validation Data Gallery

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  WB analysis using 67904-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67904-1-Ig (WIF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

• Positive WB detected in: Caco-2 cells, SW480 cells, COLO 320 cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67904-1-Ig targets WIF1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WIF1 fusion protein Ag23972
• Full Name: WNT inhibitory factor 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC018037
• Gene Symbol: WIF1
• Gene ID (NCBI): 11197
• RRID: AB_2918660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Bone; Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway; Authors - Yuanxi Leng
human	WB	Aging (Albany NY); Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma; Authors - Sen Qin