WFS1 antibody (26995-1-PBS)

Filter: AllIHCIF-PIF/ICC

IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:400 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 26995-1-AP (same clone as 26995-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

26995-1-PBS targets WFS1 in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	WFS1 fusion protein Ag25724 Predict reactive species
Full Name	Wolfram syndrome 1 (wolframin)
Calculated Molecular Weight	890 aa, 100 kDa
GenBank Accession Number	BC030130
Gene Symbol	WFS1
Gene ID (NCBI)	7466
RRID	AB_2880717
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O76024
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.