WFDC1 Polyclonal antibody

WFDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13204-1-AP

Synonyms

Prostate stromal protein ps20, PS20, ps20 growth inhibitor, WFDC1

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Validation Data Gallery

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  WB analysis of HEK-293 using 13204-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13204-1-AP (WFDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13204-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13204-1-AP (WFDC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 13204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 13204-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 13204-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13204-1-AP targets WFDC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WFDC1 fusion protein Ag3960
• Full Name: WAP four-disulfide core domain 1
• Calculated Molecular Weight: 220 aa, 24 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC029159
• Gene Symbol: WFDC1
• Gene ID (NCBI): 58189
• RRID: AB_2215895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.