WEE1 Polyclonal antibody

WEE1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14375-1-AP

Synonyms

WEE1, WEE1 homolog (S. pombe), Wee1 like protein kinase, WEE1A, Wee1A kinase, WEE1hu

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Validation Data Gallery

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Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

14375-1-AP targets WEE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WEE1 fusion protein Ag5751
• Full Name: WEE1 homolog (S. pombe)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC051831
• Gene Symbol: WEE1
• Gene ID (NCBI): 7465
• RRID: AB_2878049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).

Publications

Species	Application	Title
human	WB	J Exp Med; WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway.; Authors - Ensong Guo ; KD Validated
		Oncogene; miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells.; Authors - X-H He
mouse	WB	Exp Cell Res; Cdc14a has a role in spermatogenesis, sperm maturation and male fertility.; Authors - Zongzhuang Wen
human	WB	Exp Cell Res; NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.; Authors - Xin Zhao
human	WB	Exp Cell Res; The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.; Authors - Pan Wei
mouse	WB	Nanomedicine; The enhancement of siPLK1 penetration across BBB and its anti glioblastoma activity in vivo by magnet and transferrin co-modified nanoparticle.; Authors - Dao-Zhou Liu