CoraLite® Plus 488-conjugated WDR55 Polyclonal antibody

WDR55 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20786

Synonyms

WD repeat-containing protein 55, WD repeat domain 55

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-20786

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 WDR55 antibody (CL488-20786) at dilution of 1:200.

• 

  FC experiment of HepG2 using CL488-20786

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated WDR55 Polyclonal antibody (CL488-20786)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-20786 targets WDR55 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR55 fusion protein Ag14241
• Full Name: WD repeat domain 55
• Calculated Molecular Weight: 383 aa, 42 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC002482
• Gene Symbol: WDR55
• Gene ID (NCBI): 54853
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

WD repeat domain 55 (WDR55) also named as, FLJ20195, is a 383 amino acid protein, which contains seven WD repeats and belongs to the WD repeat WDR55 family. WDR55 localizes in the nucleus and cytoplasm. WDR55 acts as a modulator of rRNA synthesis and play a central role in organogenesis.