WDR1 Monoclonal antibody

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• KD/KO Validated

WDR1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2D11E1

Cat no : 67937-1-Ig

Synonyms

WD repeat containing protein 1, NORI-1, NORI 1, AIP1, Actin-interacting protein 1

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Validation Data Gallery

• 

  WB analysis using 67937-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67937-1-Ig (WDR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67937-1-Ig

  WB result of WDR1 antibody (67937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR1 transfected HEK-293 cells.

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  WB analysis of rat testis using 67937-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67937-1-Ig (WDR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, rat testis tissue, HEK-293 cells, U-87 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67937-1-Ig targets WDR1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WDR1 fusion protein Ag30967
• Full Name: WD repeat domain 1
• Calculated Molecular Weight: 606 aa, 66 kDa
• Observed Molecular Weight: 65-66 kDa
• GenBank Accession Number: BC030541
• Gene Symbol: WDR1
• Gene ID (NCBI): 9948
• RRID: AB_2918689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR1, which belongs to the WD repeat AIP1 family, also known as AIP1, is a 606 amino acid protein that localizes to the cytoskeleton. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WDR1 induces the disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PMID:15629458, 27557945, 29751004). WDR1 mutations may impair the ability of WDR1 to regulate cofilin-mediated actin depolymerization, and are associated with periodic fever, immunodeficiency, and thrombocytopenia syndrome (PMID: 27557945,27994071,29751004).

Publications

Species	Application	Title
human	WB	Acta Physiol (Oxf); The adipose-derived stem cell peptide ADSCP2 alleviates hypertrophic scar fibrosis via binding with pyruvate carboxylase and remodeling the metabolic landscape; Authors - Jingyun Li