WBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

WBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12030-1-AP

Synonyms

WW domain-binding protein 2, WW domain binding protein 2, WBP-2, WBP 2

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Validation Data Gallery

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  WB analysis of HEK-293 using 12030-1-AP

  WB result of WBP2 antibody (12030-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-WBP2 transfected HEK-293 cells.

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  WB analysis using 12030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12030-1-AP (WBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 12030-1-AP

  IP result of anti-WBP2 (IP:12030-1-AP, 4ug; Detection:12030-1-AP 1:300) with A375 cells lysate 3600 ug.

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  IHC staining of human breast cancer using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12030-1-AP (WBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12030-1-AP (WBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12030-1-AP (WBP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12030-1-AP (WBP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 12030-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using WBP2 antibody (12030-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A375 cells, HEK-293 cells, MCF-7 cells, MDA-MB-453s cells
• Positive IP detected in: A375 cells
• Positive IHC detected in: mouse testis tissue, human breast cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

12030-1-AP targets WBP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WBP2 fusion protein Ag2661
• Full Name: WW domain binding protein 2
• Calculated Molecular Weight: 261 aa, 28 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC010616
• Gene Symbol: WBP2
• Gene ID (NCBI): 23558
• RRID: AB_11183770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Redox Biol; WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury; Authors - Zebin Deng
mouse	WB	Eur J Immunol; Itch attenuates CD4 T cell proliferation in mice by limiting WBP2 protein stability.; Authors - Natania S Field
mouse	WB,IF	Biol Reprod; WBP2 shares a common location in mouse spermatozoa with WBP2NL/PAWP and like its descendent is a candidate mouse oocyte activating factor.; Authors - Lauren E Hamilton
rat	IF	Biol Reprod; The perforatorium and postacrosomal sheath of rat spermatozoa share common developmental origins and protein constituents†.; Authors - Nicole Protopapas
human	WB, IF	Cell Death Dis; WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.; Authors - Zhe Zheng
human,mouse	WB,IHC	Cell Death Discov; EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway.; Authors - Xuehui Wang