Various lysates were subjected to SDS PAGE followed by western blot with 67620-1-Ig (WASF3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67620-1-Ig (WASF3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 67620-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of human liver cancer using 67620-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue, human liver cancer tissue
Recommended dilution
Application
Dilution
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67620-1-Ig targets WASF3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
WASF3, also named WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with movement, invasion, and metastasis in different cancers such as breast cancer, gastric cancer, and prostate cancer. It has been shown that WASF3 levels are elevated in advanced-stage cancers compared to lower-grade tumors and normal tissue. The 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa proteins similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)
Various lysates were subjected to SDS PAGE followed by western blot with 67620-1-Ig (WASF3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67620-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse brain using 67620-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of human liver cancer using 67620-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
WASF3 Monoclonal antibody
Catalog Number
67620-1-Ig
Citations
-
Dilutions
IHC : 1:500-1:2000 IF-P : 1:200-1:800
Applications
IHC, IF-P, ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
