Vitamin D binding protein Monoclonal antibody, PBS Only

Vitamin D binding protein Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1E4D10

Cat no : 66175-1-PBS

Synonyms

GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf

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Validation Data Gallery

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  WB analysis of human testis using 66175-1-Ig (same clone as 66175-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66175-1-Ig (same clone as 66175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66175-1-Ig (same clone as 66175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66175-1-Ig (same clone as 66175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U-937 using 66175-1-Ig (same clone as 66175-1-PBS)

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

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Product Information

66175-1-PBS targets Vitamin D binding protein in Indirect ELISA, IHC, WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Vitamin D binding protein fusion protein Ag9803
• Full Name: group-specific component (vitamin D binding protein)
• Calculated Molecular Weight: 474 aa, 53 kDa
• Observed Molecular Weight: 52-58 kDa
• GenBank Accession Number: BC057228
• Gene Symbol: Vitamin D binding protein
• Gene ID (NCBI): 2638
• RRID: AB_2881570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.