Villin Monoclonal antibody, PBS Only

Villin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B7B9

Cat no : 66096-1-PBS

Synonyms

VIL1, villin 1, VIL, D2S1471, 2B7B9

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Validation Data Gallery

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  WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human small intestine using 66096-1-Ig (same clone as 66096-1-PBS)

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

  IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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Product Information

66096-1-PBS targets Villin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Villin fusion protein Ag9637
• Full Name: villin 1
• Calculated Molecular Weight: 827aa,93 kDa; 826aa,93 kDa
• Observed Molecular Weight: 93-95 kDa
• GenBank Accession Number: BC017303
• Gene Symbol: Villin
• Gene ID (NCBI): 7429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.