Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-1 Method)

EthD-I (DNA binded): Ex/Em

528/617 nm

Calcein AM：Ex/Em

494/517 nm

Application

FC

Publications

10

Cat no : PF00008

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Validation Data Gallery

• 

  Q2-UL：Cell Group with Calcein AM -, EthD-I + (dead cells)；
  Q2-LL：Cell Group with Calcein AM-, EthD-I - (cell debris)；
  Q2-LR：Cell Group with Calcein AM +, EthD-I - (live cells).

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Product Information

Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-I method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. 

This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.

Components	150T	300T
A.Calcein AM (4 mM in anhydrous DMSO)	50 uL	100 uL
B.Ethidium homodimer-1 (EthD-I) (2 mM in DMSO/H2O 1:4 (v/v))	150 uL	300 uL

Note: The test size is determined as 0.5 mL working solution for one sample in flow cytometry.

Storage

Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment. 

Protocol

Viability/Cytotoxicity cell staining kit (Calcein AM, EthD-1)

Cautions

Note that Calcein M is easy to hydrolyze, so it needs to be sealed and stored dry, and the diluted working solution needs to be prepared the same day. 

1. Fluorescent dyes all have quenching problems. Please try to avoid light during storage and use to slow down fluorescence quenching. 

2. For your safety and health, please wear lab coats and disposable gloves for operation.

Spectrum

Calcein AM：Ex/Em = 494/517 nm

EthD-I: Ex/Em = 528/617 nm (binding DNA)

Cited in Article as

PF00008, Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-1 Method), Proteintech, IL, USA

Publications

Application	Title
	Cell Stem Cell; Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids; Authors - Zheng Sun
	EMBO J; Ageing induces tissue-specific transcriptomic changes in Caenorhabditis elegans.; Authors - Xueqing Wang
	Front Endocrinol (Lausanne); Analysis by Metabolomics and Transcriptomics for the Energy Metabolism Disorder and the Aryl Hydrocarbon Receptor Activation in Male Reproduction of Mice and GC-2spd Cells Exposed to PM2.5.; Authors - Fuquan Shi
	Sci Total Environ; PM2.5 caused ferroptosis in spermatocyte via overloading iron and disrupting redox homeostasis; Authors - Jiankang Wang
	JOR Spine; Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis; Authors - Sihan He
	Nat Commun; Multiscale architecture design of 3D printed biodegradable Zn-based porous scaffolds for immunomodulatory osteogenesis; Authors - Shuang Li