VTI1B Polyclonal antibody

• Featured Product
• KD/KO Validated

VTI1B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 14495-1-AP

Synonyms

VTI1, VTI1 LIKE, Vti1 rp1, VTI1B, VTI1L, VTI1L1, VTI2

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Validation Data Gallery

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  WB analysis using 14495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 14495-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14495-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14495-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14495-1-AP

  IP result of anti-VTI1B (IP:14495-1-AP, 3ug; Detection:14495-1-AP 1:1000) with HeLa cells lysate 2500ug.

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  IHC staining of human liver cancer using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, C6 cells, NIH/3T3 cells, human liver tissue, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

14495-1-AP targets VTI1B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VTI1B fusion protein Ag5906
• Full Name: vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC003142
• Gene Symbol: VTI1B
• Gene ID (NCBI): 10490
• RRID: AB_2216160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).

Publications

Species	Application	Title
human	WB	Autophagy; The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes.; Authors - Takashi Nozawa ; KD Validated
human	WB	mBio; Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection; Authors - Sandhya Ganesan
mouse	IF	Front Cell Dev Biol; The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation; Authors - Amna Music

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had a specific band at VTI1B's predicted MW ~28 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart