VTI1A Polyclonal antibody

VTI1A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 12354-1-AP

Synonyms

Vti1-rp2, Vesicle transport v-SNARE protein Vti1-like 2, Vesicle transport through interaction with t-SNAREs homolog 1A

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Validation Data Gallery

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  WB analysis of human brain using 12354-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12354-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12354-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12354-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12354-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12354-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12354-1-AP

  IP result of anti-VTI1A (IP:12354-1-AP, 3ug; Detection:12354-1-AP 1:1000) with MCF-7 cells lysate 520ug.

• 

  IF Staining of NIH/3T3 using 12354-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 12354-1-AP (VTI1A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: human brain tissue, human heart tissue, human kidney tissue, human lung tissue, MCF-7 cells, mouse brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 3 publications below

Product Information

12354-1-AP targets VTI1A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VTI1A fusion protein Ag3018
• Full Name: vesicle transport through interaction with t-SNAREs homolog 1A (yeast)
• Calculated Molecular Weight: 217 aa, 25 kDa
• Observed Molecular Weight: 25-29 kDa
• GenBank Accession Number: BC017052
• Gene Symbol: VTI1A
• Gene ID (NCBI): 143187
• RRID: AB_2216150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). VTI1A is involved in transport between the endosome and the trans-Golgi network. The SNARE complex of STX10, STX16, VTI1A, and VAMP3 is required for MPR (mannose 6-phosphate receptors) transport from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway (PMID: 18195106). Transport from early/recycling endosomes to the TGN is mediated by the SNARE complex of STX6, STX16, VTI1A and VAMP4 (PMID: 11839770; 21807881).

Publications

Species	Application	Title
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
human	WB,IF	J Cell Biol; The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.; Authors - Laufman Orly O
human	WB,IF	J Cell Sci; The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.; Authors - Laufman Orly O
human	WB	Oncotarget; Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.; Authors - Yi Huo
human	WB,IF	Traffic; Deficiency of the Cog8 subunit in normal and CDG-derived cells impairs the assembly of the COG and Golgi SNARE complexes.; Authors - Laufman Orly O
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik