VRK2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

VRK2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12946-1-PBS

Synonyms

Vaccinia-related kinase 2, vaccinia related kinase 2, Serine/threonine-protein kinase VRK2, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 12946-1-AP (same clone as 12946-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12946-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 12946-1-AP (same clone as 12946-1-PBS)

  WB result of VRK2 antibody (12946-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VRK2 transfected HepG2 cells. This data was developed using the same antibody clone with 12946-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 12946-1-AP (same clone as 12946-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12946-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 12946-1-AP (same clone as 12946-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12946-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 12946-1-AP (same clone as 12946-1-PBS)

  IP result of anti-VRK2 (IP:12946-1-AP, 4ug; Detection:12946-1-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 12946-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12946-1-AP (same clone as 12946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12946-1-AP (VRK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12946-1-PBS in a different storage buffer formulation.

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Product Information

12946-1-PBS targets VRK2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VRK2 fusion protein Ag4019
• Full Name: vaccinia related kinase 2
• Calculated Molecular Weight: 508 aa, 58 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC027854
• Gene Symbol: VRK2
• Gene ID (NCBI): 7444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.