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KD/KO Validated

VRK2 Polyclonal antibody

Name: VRK2 Polyclonal antibody
Brand: Proteintech
SKU: 12946-1-AP

VRK2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12946-1-AP

Synonyms

Vaccinia-related kinase 2, vaccinia related kinase 2, Serine/threonine-protein kinase VRK2, EC:2.7.11.1

Validation Data Gallery

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AllWBIPIHC

WB analysis using 12946-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12946-1-AP
WB result of VRK2 antibody (12946-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VRK2 transfected HepG2 cells.
WB analysis of human liver using 12946-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 12946-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 12946-1-AP
IP result of anti-VRK2 (IP:12946-1-AP, 4ug; Detection:12946-1-AP 1:500) with K-562 cells lysate 3600ug.
IHC staining of human stomach using 12946-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12946-1-AP (VRK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12946-1-AP

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Tested Applications

Positive WB detected in	HepG2 cells, human liver tissue, mouse skeletal muscle tissue, K-562 cells, U2OS cells, BxPC-3 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 1 publications below

Product Information

12946-1-AP targets VRK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VRK2 fusion protein Ag4019
Full Name	vaccinia related kinase 2
Calculated Molecular Weight	508 aa, 58 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC027854
Gene Symbol	VRK2
Gene ID (NCBI)	7444
RRID	AB_2215681
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VRK2 antibody 12946-1-AP	Download protocol
IHC protocol for VRK2 antibody 12946-1-AP	Download protocol
IP protocol for VRK2 antibody 12946-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. Authors - Yanwei Lu View Article
human	WB	Int J Biol Sci VRK2 activates TNFα/NF-κB signaling by phosphorylating IKKβ in pancreatic cancer. Authors - Jionghuang Chen View Article
mouse	WB	BMC Med Reduced Vrk2 expression is associated with higher risk of depression in humans and mediates depressive-like behaviors in mice Authors - Mei-Yu Yin KO Validated View Article
human	WB,IHC	Cell Rep Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice Authors - Xiao-Tong Lin KD Validated View Article

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VRK2 Polyclonal antibody

VRK2 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 12946-1-AP

WB analysis of HepG2 using 12946-1-AP

WB analysis of human liver using 12946-1-AP

WB analysis of mouse skeletal muscle using 12946-1-AP

IP experiment of K-562 using 12946-1-AP

IHC staining of human stomach using 12946-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

VRK2 activates TNFα/NF-κB signaling by phosphorylating IKKβ in pancreatic cancer.

Reduced Vrk2 expression is associated with higher risk of depression in humans and mediates depressive-like behaviors in mice

Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice