VRK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

VRK2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12946-1-AP

Synonyms

Vaccinia-related kinase 2, vaccinia related kinase 2, Serine/threonine-protein kinase VRK2, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 12946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12946-1-AP

  WB result of VRK2 antibody (12946-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VRK2 transfected HepG2 cells.

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  WB analysis of human liver using 12946-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12946-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12946-1-AP

  IP result of anti-VRK2 (IP:12946-1-AP, 4ug; Detection:12946-1-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human stomach using 12946-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12946-1-AP (VRK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, mouse skeletal muscle tissue, K-562 cells, U2OS cells, BxPC-3 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

12946-1-AP targets VRK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VRK2 fusion protein Ag4019
• Full Name: vaccinia related kinase 2
• Calculated Molecular Weight: 508 aa, 58 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC027854
• Gene Symbol: VRK2
• Gene ID (NCBI): 7444
• RRID: AB_2215681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vaccinia-related kinase 2 (VRK2) is a serine/threonine kinase that plays a significant role in various cellular processes, including cell survival, proliferation, and DNA damage response. The VRK2 gene is known to produce two main splice variants: VRK2A and VRK2B, with VRK2A being the predominant form in humans. VRK2 is involved in several key biological functions. It is known to enhance cell survival by acting as an anti-apoptotic factor, which is particularly crucial in cancer biology. The overexpression of VRK2 has been linked to increased drug sensitivity in cancer cells, suggesting that this kinase may play a role in therapeutic responses. Additionally, high levels of VRK2 protein have been associated with improved survival rates in specific subgroups of astrocytomas, a type of brain tumor. (PMID: 29872222; 37943248; 24079673)

Publications

Species	Application	Title
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Int J Biol Sci; VRK2 activates TNFα/NF-κB signaling by phosphorylating IKKβ in pancreatic cancer.; Authors - Jionghuang Chen
mouse	WB	BMC Med; Reduced Vrk2 expression is associated with higher risk of depression in humans and mediates depressive-like behaviors in mice; Authors - Mei-Yu Yin ; KO Validated
human	WB,IHC	Cell Rep; Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice; Authors - Xiao-Tong Lin ; KD Validated