VRK1 Recombinant antibody

VRK1 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85598-2-RR
Clone No.242978A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, U2OS cells, MCF-7 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85598-2-RR targets VRK1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VRK1 fusion protein Ag27754 Predict reactive species
Full Name vaccinia related kinase 1
Calculated Molecular Weight 396 aa, 45 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC103761
Gene Symbol VRK1
Gene ID (NCBI) 7443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99986
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vaccinia-related kinase 1 (VRK1) is a members of the vaccinia-related kinase (VRK) family of serine/threonine kinases and are principally localized in the nucleus. VRK1 was initially described as a kinase with homology to B1R, a vaccinia virus kinase required for viral DNA replication and the early phase of infection. It has been suggested that VRK1 was overexpressed in numerous human tumors, such as lung carcinomas, hepatocellular carcinoma, breast carcinomas, head and neck squamous cell carcinoma. (PMID: 28927264, PMID: 29103766)

Protocols

Product Specific Protocols
WB protocol for VRK1 antibody 85598-2-RRDownload protocol
IF protocol for VRK1 antibody 85598-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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