VPS53 Monoclonal antibody, PBS Only

VPS53 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12C3

Cat no : 67610-1-PBS

Synonyms

FLJ10979, HCCS1, hVps53L, pp13624, VPS53

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Validation Data Gallery

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  WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

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  WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67610-1-Ig (same clone as 67610-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67610-1-Ig (same clone as 67610-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67610-1-Ig (same clone as 67610-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67610-1-Ig (VPS53 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

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Product Information

67610-1-PBS targets VPS53 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VPS53 fusion protein Ag29875
• Full Name: vacuolar protein sorting 53 homolog (S. cerevisiae)
• Calculated Molecular Weight: 832 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC029560
• Gene Symbol: VPS53
• Gene ID (NCBI): 55275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.